# Feeding physiology of M. californianus larvae in OA conditions # PI: George Waldbusser (Oregon State University) # Co-PIs: Chris Langdon, Burke Hales, & Brian Haley (Oregon State University) # Version: 16 November 2016 ========================= species ------------------------- Mytilus_californianus ========================= treatment pCO2_category aragonite_sat_category ------------------------- Control_T9 Control Control ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 20 272.04 3.245 8.19 0.750 0.763 3.000 1.250 4.250 3.278 110.559 36.000 26.333 0.939 0.030 2 24 272.04 3.245 8.19 0.682 0.763 0.917 1.042 1.958 3.278 110.559 11.000 26.333 0.939 0.030 3 24 272.04 3.245 8.19 0.857 0.763 2.667 0.958 3.625 3.278 110.559 32.000 26.333 0.939 0.030 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Ha High Low ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 40 2228.9 0.645 7.39 0.229 0.239 5.075 5.725 10.800 9.892 95.698 60.900 52.888 0.734 0.103 2 17 2228.9 0.645 7.39 0.318 0.239 3.647 3.647 7.294 9.892 95.698 43.765 52.888 0.734 0.103 3 36 2228.9 0.645 7.39 0.171 0.239 4.500 7.083 11.583 9.892 95.698 54.000 52.888 0.734 0.103 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Hb High Med-low ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 30 2175 1.313 7.55 0.320 0.317 9.200 8.000 17.200 17.178 104.628 110.400 112.733 0.924 0.164 2 40 2175 1.313 7.55 0.333 0.317 9.950 8.250 18.200 17.178 104.628 119.400 112.733 0.924 0.164 3 30 2175 1.313 7.55 0.297 0.317 9.033 7.100 16.133 17.178 104.628 108.400 112.733 0.924 0.164 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Hc High Med-High ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 35 2457.7 2.181 7.64 0.462 0.325 10.086 8.886 18.971 17.961 108.519 121.029 116.557 0.939 0.166 2 35 2457.7 2.181 7.64 0.227 0.325 8.829 8.457 17.286 17.961 108.519 105.943 116.557 0.939 0.166 3 40 2457.7 2.181 7.64 0.286 0.325 10.225 7.400 17.625 17.961 108.519 122.700 116.557 0.939 0.166 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Hd High High ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 59 2063.4 5.213 7.86 0.389 0.425 9.593 8.898 18.492 17.945 112.190 115.119 114.970 0.939 0.160 2 115 2063.4 5.213 7.86 0.415 0.425 8.983 8.026 17.009 17.945 112.190 107.791 114.970 0.939 0.160 3 30 2063.4 5.213 7.86 0.472 0.425 10.167 8.167 18.333 17.945 112.190 122.000 114.970 0.939 0.160 ========================= treatment pCO2_category aragonite_sat_category ------------------------- La Low Low ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 27 219 0.499 7.84 0.524 0.605 5.741 4.444 10.185 9.271 91.220 68.889 57.866 0.576 0.102 2 36 219 0.499 7.84 0.625 0.605 5.639 4.250 9.889 9.271 91.220 67.667 57.866 0.576 0.102 3 23 219 0.499 7.84 0.667 0.605 3.087 4.652 7.739 9.271 91.220 37.043 57.866 0.576 0.102 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Lb Low Med-low ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 35 197.36 1.088 8.03 0.773 0.689 8.086 5.914 14.000 14.351 102.714 97.029 100.378 0.903 0.140 2 35 197.36 1.088 8.03 0.643 0.689 7.457 6.114 13.571 14.351 102.714 89.486 100.378 0.903 0.140 3 29 197.36 1.088 8.03 0.650 0.689 9.552 5.931 15.483 14.351 102.714 114.621 100.378 0.903 0.140 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Lc Low Med-High ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 32 241.46 1.87 8.11 0.469 0.624 7.406 6.500 13.906 14.064 107.513 88.875 89.625 0.938 0.131 2 27 241.46 1.87 8.11 0.625 0.624 7.333 7.037 14.370 14.064 107.513 88.000 89.625 0.938 0.131 3 36 241.46 1.87 8.11 0.778 0.624 7.667 6.250 13.917 14.064 107.513 92.000 89.625 0.938 0.131 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Ld Low High ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 92 191.12 4.582 8.35 0.250 0.546 6.707 5.293 12.000 12.812 111.816 80.478 87.607 0.939 0.115 2 56 191.12 4.582 8.35 0.808 0.546 7.304 4.589 11.893 12.812 111.816 87.643 87.607 0.939 0.115 3 83 191.12 4.582 8.35 0.581 0.546 7.892 6.651 14.542 12.812 111.816 94.699 87.607 0.939 0.115 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Mha Med-High Low ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 29 873.75 0.505 7.54 0.385 0.366 4.690 7.276 11.966 9.962 91.445 56.276 55.464 0.584 0.109 2 27 873.75 0.505 7.54 0.381 0.366 5.296 5.704 11.000 9.962 91.445 63.556 55.464 0.584 0.109 3 25 873.75 0.505 7.54 0.333 0.366 3.880 3.040 6.920 9.962 91.445 46.560 55.464 0.584 0.109 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Mhb Med-High Med-low ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 36 772.87 1.137 7.77 0.515 0.536 9.667 7.528 17.194 16.587 103.189 116.000 104.040 0.909 0.161 2 36 772.87 1.137 7.77 0.571 0.536 8.583 9.222 17.806 16.587 103.189 103.000 104.040 0.909 0.161 3 25 772.87 1.137 7.77 0.520 0.536 7.760 7.000 14.760 16.587 103.189 93.120 104.040 0.909 0.161 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Mhc Med-High Med-High ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 30 803.45 2.327 7.88 0.615 0.562 9.867 7.567 17.433 17.087 108.904 118.400 122.693 0.939 0.157 2 25 803.45 2.327 7.88 0.500 0.562 9.440 6.120 15.560 17.087 108.904 113.280 122.693 0.939 0.157 3 30 803.45 2.327 7.88 0.571 0.562 11.367 6.900 18.267 17.087 108.904 136.400 122.693 0.939 0.157 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Mhd Med-High High ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 29 767.64 4.691 8.05 0.571 0.513 10.310 10.828 21.138 13.793 111.887 123.724 123.161 0.939 0.123 2 25 767.64 4.691 8.05 0.423 0.513 4.480 7.360 11.840 13.793 111.887 53.760 123.161 0.939 0.123 3 30 767.64 4.691 8.05 0.545 0.513 1.000 7.400 8.400 13.793 111.887 12.000 123.161 0.939 0.123 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Mla Med-low Low ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 27 715.68 0.31 7.478 0.412 0.526 1.741 7.852 9.593 6.762 81.011 20.889 7.068 0.180 0.083 2 38 715.68 0.31 7.478 0.697 0.526 0.026 2.553 2.579 6.762 81.011 0.316 7.068 0.180 0.083 3 26 715.68 0.31 7.478 0.471 0.526 0.000 8.115 8.115 6.762 81.011 0.000 7.068 0.180 0.083 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Mlb Med-low Med-low ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 48 437.35 1.028 7.84 0.484 0.587 1.188 8.229 9.417 10.179 102.078 14.250 7.910 0.893 0.100 2 28 437.35 1.028 7.84 0.636 0.587 0.643 9.536 10.179 10.179 102.078 7.714 7.910 0.893 0.100 3 34 437.35 1.028 7.84 0.641 0.587 0.147 10.794 10.941 10.179 102.078 1.765 7.910 0.893 0.100 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Mlc Med-low Med-High ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 20 396.28 2.355 8.04 0.563 0.561 0.500 11.400 11.900 12.467 108.972 6.000 3.200 0.939 0.114 2 20 396.28 2.355 8.04 0.455 0.561 0.000 11.900 11.900 12.467 108.972 0.000 3.200 0.939 0.114 3 20 396.28 2.355 8.04 0.667 0.561 0.300 13.300 13.600 12.467 108.972 3.600 3.200 0.939 0.114 ========================= treatment pCO2_category aragonite_sat_category ------------------------- Mld Med-low High ========================= replicate n_evaluated pCO2 aragonite_sat pH pcnt_feeding_init pcnt_feeding_init_mean red_beads_mean yellow_beads_mean gut_fullness gut_fullness_mean size_est ingest_rate_hrly ingest_rate_hrly_mean size_std_ingestion_rates size_std_gut_fullness ------------------------- 1 34 384.26 4.758 8.23 0.436 0.502 0.000 7.794 7.794 9.242 111.930 0.000 0.148 0.939 0.083 2 27 384.26 4.758 8.23 0.500 0.502 0.037 11.815 11.852 9.242 111.930 0.444 0.148 0.939 0.083 3 25 384.26 4.758 8.23 0.571 0.502 0.000 8.080 8.080 9.242 111.930 0.000 0.148 0.939 0.083